ISO 21446:2019
Infant formula and adult nutritionals — Determination of trans and total (cis + trans) vitamin K1 content — Normal phase HPLC
OVERVIEW
This document specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K1 in infant, pediatric and adult nutritionals using normal phase (NP) high-performance liquid chromatography (HPLC) with post-column reduction and fluorescence detection. The method demonstrated good linearity over a standard range of ~2 µg/l to 80 µg/l trans vitamin K1, and the limit of quantification (LOQ) was estimated to be 0,4 µg/l for standards and 0,09 µg/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.
COMMENTS
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PRODUCT DETAILS
| Status | Current | 
|---|---|
| Edition | 2019 | 
| No. of Pages | 20 | 
| ICS Classification | 67.050 General methods of tests and analysis for food products | 
                        
| Committee | ISO/TC 34 | 
| Available for Purchase | For sale in Singapore only | 
| Adoption | ISO |